Review



pcpgrich lacz  (InvivoGen)


Bioz Verified Symbol InvivoGen is a verified supplier
Bioz Manufacturer Symbol InvivoGen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    InvivoGen pcpgrich lacz
    Pcpgrich Lacz, supplied by InvivoGen, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcpgrich+lacz/pCpGrich-mcs/pmc10213191-243-88-83
    Average 90 stars, based on 5 article reviews
    pcpgrich lacz - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: 704/DNA vaccines leverage cytoplasmic DNA stimulation to promote anti-HIV neutralizing antibody production in mice and strong immune response against alpha-fetoprotein in non-human primates
    Article Snippet: .. The pCMV-LacZ plasmid (Clontech, St Germain en Laye, France), encodingβ-Gal under the control of the human cytomegalovirus (CMV) immediate-early gene promoter, was used as antigen. pCMV-LacZ-miR133a-3p and pCMV-LacZ-miR142a-3p were constructed by cloning four miRNA 133a-3p targeting sequences (forward, cagctggttgaggggaccaaa; reverse, tttggtcccctcaaccagctg) or four miRNA 142a-3p targeting sequences (forward, tccataaagtaggaaacactaca; reverse, tgtagtgtttcctactttatgga) into the 3′ UTR of the LacZ gene, which inhibit respectively the expression on muscular fibers or DCs. pCpGrich-LacZ was constructed by cloning the LacZ gene from pCpGfree-LacZ into the pCpGrich-mcs backbone (InvivoGen, San Diego, CA, USA). pCpGrich-LacZ contains 88 CpG dinucleotides motifs, whereas pCpGfree-LacZ has none. ..

    Control:

    Article Title: 704/DNA vaccines leverage cytoplasmic DNA stimulation to promote anti-HIV neutralizing antibody production in mice and strong immune response against alpha-fetoprotein in non-human primates
    Article Snippet: .. The pCMV-LacZ plasmid (Clontech, St Germain en Laye, France), encodingβ-Gal under the control of the human cytomegalovirus (CMV) immediate-early gene promoter, was used as antigen. pCMV-LacZ-miR133a-3p and pCMV-LacZ-miR142a-3p were constructed by cloning four miRNA 133a-3p targeting sequences (forward, cagctggttgaggggaccaaa; reverse, tttggtcccctcaaccagctg) or four miRNA 142a-3p targeting sequences (forward, tccataaagtaggaaacactaca; reverse, tgtagtgtttcctactttatgga) into the 3′ UTR of the LacZ gene, which inhibit respectively the expression on muscular fibers or DCs. pCpGrich-LacZ was constructed by cloning the LacZ gene from pCpGfree-LacZ into the pCpGrich-mcs backbone (InvivoGen, San Diego, CA, USA). pCpGrich-LacZ contains 88 CpG dinucleotides motifs, whereas pCpGfree-LacZ has none. ..

    Construct:

    Article Title: 704/DNA vaccines leverage cytoplasmic DNA stimulation to promote anti-HIV neutralizing antibody production in mice and strong immune response against alpha-fetoprotein in non-human primates
    Article Snippet: .. The pCMV-LacZ plasmid (Clontech, St Germain en Laye, France), encodingβ-Gal under the control of the human cytomegalovirus (CMV) immediate-early gene promoter, was used as antigen. pCMV-LacZ-miR133a-3p and pCMV-LacZ-miR142a-3p were constructed by cloning four miRNA 133a-3p targeting sequences (forward, cagctggttgaggggaccaaa; reverse, tttggtcccctcaaccagctg) or four miRNA 142a-3p targeting sequences (forward, tccataaagtaggaaacactaca; reverse, tgtagtgtttcctactttatgga) into the 3′ UTR of the LacZ gene, which inhibit respectively the expression on muscular fibers or DCs. pCpGrich-LacZ was constructed by cloning the LacZ gene from pCpGfree-LacZ into the pCpGrich-mcs backbone (InvivoGen, San Diego, CA, USA). pCpGrich-LacZ contains 88 CpG dinucleotides motifs, whereas pCpGfree-LacZ has none. ..

    Cloning:

    Article Title: 704/DNA vaccines leverage cytoplasmic DNA stimulation to promote anti-HIV neutralizing antibody production in mice and strong immune response against alpha-fetoprotein in non-human primates
    Article Snippet: .. The pCMV-LacZ plasmid (Clontech, St Germain en Laye, France), encodingβ-Gal under the control of the human cytomegalovirus (CMV) immediate-early gene promoter, was used as antigen. pCMV-LacZ-miR133a-3p and pCMV-LacZ-miR142a-3p were constructed by cloning four miRNA 133a-3p targeting sequences (forward, cagctggttgaggggaccaaa; reverse, tttggtcccctcaaccagctg) or four miRNA 142a-3p targeting sequences (forward, tccataaagtaggaaacactaca; reverse, tgtagtgtttcctactttatgga) into the 3′ UTR of the LacZ gene, which inhibit respectively the expression on muscular fibers or DCs. pCpGrich-LacZ was constructed by cloning the LacZ gene from pCpGfree-LacZ into the pCpGrich-mcs backbone (InvivoGen, San Diego, CA, USA). pCpGrich-LacZ contains 88 CpG dinucleotides motifs, whereas pCpGfree-LacZ has none. ..

    Expressing:

    Article Title: 704/DNA vaccines leverage cytoplasmic DNA stimulation to promote anti-HIV neutralizing antibody production in mice and strong immune response against alpha-fetoprotein in non-human primates
    Article Snippet: .. The pCMV-LacZ plasmid (Clontech, St Germain en Laye, France), encodingβ-Gal under the control of the human cytomegalovirus (CMV) immediate-early gene promoter, was used as antigen. pCMV-LacZ-miR133a-3p and pCMV-LacZ-miR142a-3p were constructed by cloning four miRNA 133a-3p targeting sequences (forward, cagctggttgaggggaccaaa; reverse, tttggtcccctcaaccagctg) or four miRNA 142a-3p targeting sequences (forward, tccataaagtaggaaacactaca; reverse, tgtagtgtttcctactttatgga) into the 3′ UTR of the LacZ gene, which inhibit respectively the expression on muscular fibers or DCs. pCpGrich-LacZ was constructed by cloning the LacZ gene from pCpGfree-LacZ into the pCpGrich-mcs backbone (InvivoGen, San Diego, CA, USA). pCpGrich-LacZ contains 88 CpG dinucleotides motifs, whereas pCpGfree-LacZ has none. ..



    Similar Products

    90
    InvivoGen pcpgrich lacz
    Pcpgrich Lacz, supplied by InvivoGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcpgrich+lacz/pCpGrich-mcs/pmc10213191-243-88-83
    Average 90 stars, based on 1 article reviews
    pcpgrich lacz - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results